Analysis of Total Terpenoids from Maniltoa Grandiflora (A. Gray) Scheff Leaves Using TLC and HPLC Methods

Jhon Patar Sinurat; Visensius Krisdianilo; Reh Malem br Karo; Rinaldo Berutu

doi:10.33019/jstk.v2i2.1976

Jhon Patar Sinurat Institut Kesehatan Medistra Lubuk Pakam
Visensius Krisdianilo Departement of Medical Laboratory Technology, Faculty of Pharmacy, Institut Kesehatan Medistra Lubuk Pakam, Sudirman street No. 38, Deli Serdang, North Sumatera, 20512. Indonesia
Reh Malem br Karo Departement of Pharmacy, Faculty of Medicine, Universitas Prima Indonesia, 01 Belanga Street, Simpang Ayahanda, Medan, 20118, Indonesia
Rinaldo Berutu Departement of Medical Laboratory Technology, Faculty of Pharmacy, Institut Kesehatan Medistra Lubuk Pakam, Sudirman street No. 38, Deli Serdang, North Sumatera, 20512. Indonesia

DOI: https://doi.org/10.33019/jstk.v2i2.1976

Abstract

Terpenoids screening were carried out using Liebermann Burchard and Salkowski reagent on the extract of Saputangan leaves. It showed that the leaves contained terpenoid compounds with appeared of a reddish brown ring in the extract and a reddish brown stain appeared on the TLC plate tested with 1% CeSO₄ reagent in 10% H₂SO₄. The macerate of saputangan leaves processed separation using the partition method (Liquid-liquid Extraction). Extracts dissolved with methanol were partitioned with n-Hexane and then partitioned between aquadest and ethyl acetate in a ratio of 1: 1 to obtain 50 g of total terpenoids. Furthermore, TLC analysis was performed on total terpenoids using n-hexane: ethyl acetate (80:20 v/v) solvent to obtain 11 separate stains on the TLC plate with different Rf each. Analysis was enhanced in HPLC using 100% acetonitrile and 0.1% phosphoric acid at a wavelength of 210 nm, a flow rate of 0.500 mL/min and eluted for 30 minutes. Based on the HPLC results, there were 25 peaks which indicated the presence of total terpenoid compounds with the highest peak being peak no. 8 (ret.time's 6.234, area's 8503532 and height's 276032), peak no. 9 (ret.time's 6.674, area's 3322572 and height's 141859) and peak no. 10 (ret.time's 7.288, area's 2758231 and height's 103927)

Keywords: Terpenoids, HPLC, TLC and Rf

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