Testing The Effectiveness of Biological Agents in Controlling Disease-Causing Pathogens In Vitro

Uji Efektivitas Agen Hayati Dalam Pengendalian Patogen Penyebab Penyakit Secara In Vitro

Authors

  • Nur Febriansyah Rahmanda Universitas Bangka Belitung
  • Miranda Miranda Universitas Bangka Belitung
  • Muniroh Muniroh Universitas Bangka Belitung
  • Indri Riyana Universitas Bangka Belitung
  • Jumita Jumita Universitas Bangka Belitung
  • Gholi Wedana Nursyah Universitas Bangka Belitung
  • Gen Adi Wisanggeni Universitas Bangka Belitung https://orcid.org/0000-0003-4601-8874

DOI:

https://doi.org/10.33019/t0622450

Keywords:

Agen Hayati, Antagonisme, Biokontrol, Gliocladium, Trichoderma

Abstract

Alternative plant disease control can be carried out using biological agents in an effort to reduce chemical pesticides. The antagonistic mechanism possessed by biological agents has been widely used as an effective and environmentally friendly alternative. Biological agents that are often used for biocontrol include Trichoderma sp. and Gliocladium sp.. This experiment aims to determine the comparative antagonism of Trichoderma spp. and Gliocladium biological agents against Curvularia spp. and Fusarium spp. This experiment was conducted to test the antagonism of biological agents. The experimental factors tested to determine the inhibitory effect were Trichoderma sp. and Gliocladium sp. isolates. Next, pure cultures of Trichoderma sp. and Gliocladium spp., which were 7 days old, were inoculated into the medium to observe the contact between Trichoderma sp. and Curvularia sp, Trichoderma sp. and Fusarium sp., Gliocladium sp and Curvularia sp., and Gliocladium sp and Fusarium sp. The antagonistic test results showed that the Trichoderma sp. biological agent treatment was able to optimally inhibit the growth of Curvularia sp. and Fusarium sp. The Gliocladium sp. treatment was able to inhibit the growth of Fusarium sp., but was less optimal in inhibiting the growth of Curvularia sp.

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Author Biographies

  • Nur Febriansyah Rahmanda, Universitas Bangka Belitung

    Program Studi Agroteknologi, Fakultas Pertanian Perikanan dan Kelautan

  • Miranda Miranda, Universitas Bangka Belitung

    Program Studi Agroteknologi, Fakultas Pertanian Perikanan dan Kelautan

  • Muniroh Muniroh, Universitas Bangka Belitung

    Program Studi Agroteknologi, Fakultas Pertanian Perikanan dan Kelautan

  • Indri Riyana, Universitas Bangka Belitung

    Program Studi Agroteknologi, Fakultas Pertanian Perikanan dan Kelautan

  • Jumita Jumita, Universitas Bangka Belitung

    Program Studi Agroteknologi, Fakultas Pertanian Perikanan dan Kelautan

  • Gholi Wedana Nursyah, Universitas Bangka Belitung

    Program Studi Agroteknologi, Fakultas Pertanian Perikanan dan Kelautan

  • Gen Adi Wisanggeni, Universitas Bangka Belitung

    Program Studi Agroteknologi, Fakultas Pertanian Perikanan dan Kelautan

References

Abbas, A., Zheng, H., Zuñiga, C., & Moya-elizondo, E. A. (2022). Trichoderma spp . Genes Involved in the Biocontrol Activity Against Rhizoctonia solani. Front. Microbiol, 13(May), 1–22. https://doi.org/10.3389/fmicb.2022.884469

Al-askar, A. A., Saber, W. I. A., Ghoneem, K. M., Hafez, E. E., & Ibrahim, A. A. (2021). Crude Citric Acid of Trichoderma asperellum : Tomato Growth.

Al-surhanee, A. A. (2022). Saudi Journal of Biological Sciences Protective role of antifusarial eco-friendly agents ( Trichoderma and salicylic acid ) to improve resistance performance of tomato plants. Saudi Journal of Biological Sciences, 29(4), 2933–2941. https://doi.org/10.1016/j.sjbs.2022.01.020

Anadiasthy, I. S., Bimantara, A., Alifianto, L. G., & Wicaksono, D. P. (2025). Antagonism Test of Trichoderma harzianum and Gliocladium sp . in the Golden Tricho Product Against Fusarium sp . Prosiding Seminar Nasional Penelitian Dan Pengabdian Kepada Masyarakat, 3, 518–531.

Asad, S. A. (2022). Mechanisms of action and biocontrol potential of Trichoderma against fungal plant diseases - A review. Ecological Complexity, 49(June 2021), 100978. https://doi.org/10.1016/j.ecocom.2021.100978

Ayaz, M., Ali, F., Yu, X., Yu, Q., Zhao, J., Yu, J., Qi, R., & Huang, W. (2023). Bacterial and Fungal Biocontrol Agents for Plant Disease Protection: Journey from Lab to Field, Current Status, Challenges, and Global Perspectives. Molecules, 28(6375), 1–24. https://doi.org/https://doi.org/10.3390/molecules28186735

Baiyee, B., Pornsuriya, C., Ito, S., & Sunpapao, A. (2019). Trichoderma spirale T76-1 displays biocontrol activity against leaf spot on lettuce ( Lactuca sativa L .) caused by Corynespora cassiicola or Curvularia aeria. Biological Control, 129(June 2018), 195–200. https://doi.org/10.1016/j.biocontrol.2018.10.018

Beyari, E. A. (2025). Alternatives to chemical pesticides: the role of microbial biocontrol agents in phytopathogen management: a comprehensive review. Journal of Plant Pathology, 107(1), 291–314. https://doi.org/10.1007/s42161-024-01808-8

Darmanto, K., Lihawa, M., Husain, I., & Pulogu, S. I. (2025). Uji Antagonis Agen Hayati Trichoderma Sp. Terhadap Colletotrfichum Capsici Dan Fusarium Sp. Dari Tanaman Cabai. JATT, 14(1), 52–66.

Elnahal, A. S. M., El-Saadony, M. T., Saad, A. M., Desoky, E.-S. M., El-Tahan, A. M., Rady, M. M., AbuQamar, S. F., & El-Tarabily, K. A. (2022). The use of microbial inoculants for biological control, plant growth promotion, and sustainable agriculture: A review. European Journal of Plant Pathology, 162(4), 759–792. https://doi.org/10.1007/s10658-021-02393-7

Fadiji, A. E., & Babalola, O. O. (2020). Elucidating Mechanisms of Endophytes Used in Plant Protection and Other Bioactivities With Multifunctional Prospects. Front. Bioeng and Biotechnol, 8(May), 1–20. https://doi.org/10.3389/fbioe.2020.00467

Fahad, S., Saud, S., Akhter, A., Ahsan, A., Hassan, S., Battaglia, M., Adnan, M., Wahid, F., Datta, R., Babur, E., Danish, S., & Zarei, T. (2021). Journal of the Saudi Society of Agricultural Sciences Bio-based integrated pest management in rice : An agro-ecosystems friendly approach for agricultural sustainability. Journal of the Saudi Society of Agricultural Sciences, 20(2), 94–102. https://doi.org/10.1016/j.jssas.2020.12.004

Fardhani, D. M., Fuadiyah, D. A., Susila, W. A., & Afifah, I. (2024). Inhibition of Gliocladium sp against plant pathogenic fungus and their exoenzyme activity. International Journal of Health Science and Technology, 6(1), 45–54. https://doi.org/https://doi.org/10.31101/ijhst.v6i1.3312

Fenta, L., & Mekonnen, H. (2024). Microbial Biofungicides as a Substitute for Chemical Fungicides in the Control of Phytopathogens : Current Perspectives and. Scientifica, 2024(November), 1–22. https://doi.org/10.1155/2024/5322696

Fontana, D. C., Paula, S. De, Torres, A. G., Hugo, V., Souza, M. De, Pascholati, F., Schmidt, D., & Neto, D. D. (2021). Endophytic Fungi : Biological Control and Induced Resistance to Phytopathogens and Abiotic Stresses. Pathogens Review, 10(570), 1–28. https://doi.org/https://doi.org/10.3390/pathogens10050570

Gualtieri, L., Monti, M. M., Mele, F., Russo, A., Pedata, P. A., & Ruocco, M. (2022). Volatile Organic Compound ( VOC ) Profiles of Different Trichoderma Species and Their Potential Application. Journal of Fungi Article, 8(989), 1–15. https://doi.org/https://doi.org/10.3390/jof8100989

Guzman, Paulina, K., Ajay, Santos-villalobos, S. D. L., Parra-cota, F. I., Orozco-mosqueda, M. C., Fadiji, A. E., Hyder, S., Babalola, O. O., & Santoyo, G. (2023). Trichoderma Species : Our Best Fungal Allies in the Biocontrol of Plant Diseases — A Review. Plants Review, 12(432), 1–35. https://doi.org/https://doi.org/10.3390/ plants12030432

Harahap, M. S., Siregar, E. J., Lubis, M. H., Harahap, H., Lubis, J. R., Muhammad, T., & Gunung, R. (2024). Optimalisasi Strategi Pengendalian Bercak Daun Curvularia sp. pada Pembibitan Kelapa Sawit di Desa Binanga. Jumas : Jurnal Masyarakat, 03(1995), 73–79. https://doi.org/https://doi.org/10.54209/jumas.v3i02.93 spesies

Irmawatie, L., Adviany, I., Hamdani, D., Nurdin, R., Suswana, S., Ginger, E., Ginger, E., Ginger, R., & Biostimulant, A. (2025). Effects of Gliocladium sp . on Suppression of the Intensity of Scab Disease ( Streptomyces scabies Lambert and Loria ) and the Quality of Potato Seeds ( Solanum tuberosum L .). Central Research Institute for Agriculture (CeRIA), 19(1), 9–14. https://doi.org/10.59651/ccria

Kalimutu, K., Bagus, I., Mahardika, K., Agung, A., & Putri, S. (2020). SEAS ( Sustainable Environment Agricultural Science ) Antagonism Test of Trichoderma atroviride and Gliocladium sp . Bali Local Isolates As a Disease Control of Blendok Disease ( Botryodiplodia theobromae ) in Grapefruit ( Citrus grandis L . Osbeck ). SEAS (Sustainable Environment Agricultural Science), 04(02), 102–110. https://doi.org/http://dx.doi.org/10.22225/seas.4.2.2311.102-110

Khalimi, K., Wayan, N., & Leana, A. (2025). Antifungal Activity of Gliocladium viride against Fusarium oxysporum. Jurnal Ilmu Pertanian Indonesia (JIPI), 30(April), 268–276. https://doi.org/10.18343/jipi.30.2.268

Lahlali, R., Ezrari, S., Radouane, N., Kenfaoui, J., & Esmaeel, Q. (2022). Biological Control of Plant Pathogens : A Global Perspective. Microorganisms Review, 10(596), 1–33. https://doi.org/https://doi.org/10.3390/microorganisms10030596

Li, J., Hu, M., Xue, Y., Chen, X., Lu, G., & Zhang, L. (2020). microorganisms Screening , Identification and E ffi cacy Evaluation of Antagonistic Bacteria for Biocontrol of Soft Rot Disease Caused by Dickeya zeae. Microorganisms, 8(697), 2–19. https://doi.org/10.3390/microorganisms8050697

Malik, A. F., Siagian, R., & Diyasti, F. (2022). Penghambatan Gliocladium Sp. Cordo. Terhadap Phytophthora Capsici Leonian. Penyebab Penyakit Busuk Pangkal Batang Lada Serta Bentuk Formulasinya. AGROSCRIPT Journal of Applied Agricultural Sciences, 4(1), 39–47. https://doi.org/https://doi.org/10.36423/agroscript.v4i1.972

Mukaromah, B. D., Bahri, S., & Indahsari, W. M. N. (2025). Exploration and Identification of Gliocladium sp as Biological Agent from the Rhizosphere of Tomato. Jurnal Ilmiah Biologi Eksperimen Dan Keanekaragaman Hayati (J-BEKH), 12(1), 1–12. https://doi.org/https://doi.org/10.23960/jbekh.v12i1.390

Negi, R., Sharma, B., Kaur, S., Kaur, T., Khan, S. S., Kumar, S., Ramniwas, S., Rustagi, S., Singh, S., Rai, A. K., Kour, D., & Thakur, N. (2023). Microbial antagonists : diversity , formulation and applications for management of pest – pathogens. Egyptian Journal of Biological Pest Control. https://doi.org/10.1186/s41938-023-00748-2

Oyesola, O. L., Kinge, R. T., & Obembe, O. O. (2025). Trichoderma: A Review of its Mechanisms of Action in Plant Sustainable Disease Control. IOP Conference Series: Earth and Environmental Science, 1492(1), 12008. https://doi.org/10.1088/1755-1315/1492/1/012008

Poveda, J., Millen, M. R., & Bailey, A. M. (2024). Analysis of Trichoderma as an effective biological control agent against the honey fungus ( Armillaria spp .). Biological Control, 188(August 2023), 105424. https://doi.org/10.1016/j.biocontrol.2023.105424

Saad, A. M., Soliman, S. M., Salem, H. M., Ahmed, A. I., Babalghith, A. O., & Elrys, A. S. (2022). Plant growth-promoting microorganisms as biocontrol agents of plant diseases : Mechanisms , challenges and future perspectives. Frontiers in Plant Science, 13(October), 1–19. https://doi.org/10.3389/fpls.2022.923880

Shaili, S. J., & Kabiraj, U. K. (2025). Fungal Biocontrol in Agriculture : A Sustainable Alternative to Chemical Pesticides – A Comprehensive Review. World Journal of Advanced Research and Reviews, 26(01), 2305–2316. https://doi.org/https://doi.org/10.30574/wjarr.2025.26.1.0732 Abstract

Shekhar, C., Khosya, R., Thakur, K., Mahajan, D., Kumar, R., Kumar, S., & Sharma, A. K. (2024). A systematic review of pesticide exposure , associated risks , and long-term human health impacts. Toxicology Reports, 13(November), 101840. https://doi.org/10.1016/j.toxrep.2024.101840

Sofian, Sopialena, & Aprilia, R. (2025). Antagonistic Test of Endophytic Fungi from Black Pepper ( Piper nigrum L .) against Fusarium oxysporum the Main Cause of Fusarium Wilt in Chili Plants ( Capsicum annuum L .). JAPSI (Journal of Agriprecision and Social Impact), 2(3), 368–387. https://doi.org/10.62793/japsi.v2i3.75

Sopialena, Nadira, N., & Arwita, P. (2024). Antagonist Test of Trichoderma sp and Gliocladium sp Againts Fungal Pathogens That Cause Diseases on Tomato Plant. Jurnal Agroekoteknologi Tropika Lembab, 7(1), 78–84. https://doi.org/DOI.210.35941/JATL

Syarifah, S. M., Sari, O. P., & Bimantara, A. (2024). Isolat Trichoderma sp . Asal Rizosfer Bambu dari Kecamatan Kedu , Kabupaten Temanggung ( Pathogen Biological Control of Fusarium oxysporum f . sp . capsica using Isolated Trichoderma sp . of Bamboo Rhizosphere from Kedu District , Temanggung Regency ). Jurnal Ilmu Pertanian Indonesia (JIPI), 29(3), 454–460. https://doi.org/10.18343/jipi.29.3.454

Thambugala, K. M., Daranagama, D. A., & Phillips, A. J. L. (2020). Fungi vs . Fungi in Biocontrol : An Overview of Fungal Antagonists Applied Against Fungal Plant Pathogens. Front. Cell. Infect. Microbiol., 10(November), 1–19. https://doi.org/10.3389/fcimb.2020.604923

Tudi, M., Ruan, H. D., Wang, L., Lyu, J., Sadler, R., Connell, D., & Chu, C. (2021). Agriculture Development , Pesticide Application and Its Impact on the Environment. International Journal of Environmental Research and Public Health, 18(1112), 1–23. https://doi.org/https://doi.org/10.3390/ijerph18031112

Tyskiewicz, R., Nowak, A., & Ozimek, E. (2022). Trichoderma : The Current Status of Its Application in Agriculture for the Biocontrol of Fungal Phytopathogens and Stimulation of Plant Growth. Molecular Sciences, 23(2329), 1–28.

Vicente, I., Baroncelli, R., Bernardi, R., Puntoni, G., Hermosa, R., Monte, E., Vannacci, G., & Sarrocco, S. (2020). Combined Comparative Genomics and Gene Expression Analyses Provide Insights into the Terpene Synthases Inventory in Trichoderma. Microorganisms Article, 8(1603), 1–20. https://doi.org/doi:10.3390/microorganisms8101603

Yusmar, M., Aslamil, M., & Siti, Z. (2023). Uji Pelet Biofungisida Trichoderma Harzianum Mengandung Beberapa Bahan Alami terhadap Curvularia Sp . secara In Vitro. Prosiding Seminar Nasional Pembangunan Dan Pendidikan Vokasi Pertanian, 507–519. https://doi.org/https://doi.org/10.47687/snppvp.v4i1.674

Zhang, C., Wang, W., Xue, M., Liu, Z., Zhang, Q., & Hou, J. (2021). The Combination of a Biocontrol Agent Trichoderma asperellum SC012 and Hymexazol Reduces the Effective Fungicide Dose to Control Fusarium Wilt in Cowpea.

Zhang, Y., Xiao, J., Yang, K., Wang, Y., Tian, Y., & Id, Z. L. (2022). Transcriptomic and metabonomic insights into the biocontrol mechanism of Trichoderma asperellum M45a against watermelon. 1–19.

Zhou, W., Li, M., & Achal, V. (2025). A comprehensive review on environmental and human health impacts of chemical pesticide usage. Emerging Contaminants, 11(1), 100410. https://doi.org/10.1016/j.emcon.2024.100410

Zin, N. A., & Badaluddin, N. A. (2020). Annals of Agricultural Sciences Biological functions of Trichoderma spp . for agriculture applications. Annals of Agricultural Sciences, 65(2), 168–178. https://doi.org/10.1016/j.aoas.2020.09.003

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Published

27-10-2026

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Vol. 12 No. 2 (2026): Enviagro: Jurnal Pertanian dan Lingkungan

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Articles research

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Copyright (c) 2026 Nur Febriansyah Rahmanda, Miranda Miranda, Muniroh Muniroh, Indri Riyana, Jumita Jumita, Gholi Wedana Nursyah, Gen Adi Wisanggeni

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How to Cite

Rahmanda, N. F., Miranda, M., Muniroh, M., Riyana, . I., Jumita, J., Nursyah, G. W., & Wisanggeni, G. A. (2026). Testing The Effectiveness of Biological Agents in Controlling Disease-Causing Pathogens In Vitro: Uji Efektivitas Agen Hayati Dalam Pengendalian Patogen Penyebab Penyakit Secara In Vitro. Enviagro: Jurnal Pertanian Dan Lingkungan, 12(2), 74-82. https://doi.org/10.33019/t0622450

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